Immunofluorescence assay for detection of the nucleocapsid antigen of the severe acute respiratory syndrome (SARS)-associated coronavirus in cells derived from throat wash samples of patients with SARS
Identifieur interne : 004E09 ( Main/Exploration ); précédent : 004E08; suivant : 004E10Immunofluorescence assay for detection of the nucleocapsid antigen of the severe acute respiratory syndrome (SARS)-associated coronavirus in cells derived from throat wash samples of patients with SARS
Auteurs : I.-Jung Liu [Taïwan] ; Pei-Jer Chen [Taïwan] ; Shiou-Hwei Yeh [Taïwan] ; Yu-Ping Chiang [Taïwan] ; Li-Min Huang [Taïwan] ; Ming-Fu Chang [Taïwan] ; Shey-Ying Chen [Taïwan] ; Pan-Chyr Yang [Taïwan] ; Shan-Chwen Chang [Taïwan] ; Wei-Kung Wang [Taïwan]Source :
- Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2005.
Descripteurs français
- KwdFr :
- Antigènes viraux (analyse), Humains, Nucléocapside (analyse), Pharynx (anatomopathologie), Pharynx (virologie), Syndrome respiratoire aigu sévère (anatomopathologie), Syndrome respiratoire aigu sévère (diagnostic), Technique d'immunofluorescence, Virus du SRAS (immunologie), Virus du SRAS (isolement et purification).
- MESH :
- analyse : Antigènes viraux, Nucléocapside.
- anatomopathologie : Pharynx, Syndrome respiratoire aigu sévère.
- diagnostic : Syndrome respiratoire aigu sévère.
- immunologie : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Pharynx.
- Pascal (Inist)
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
- Antigen, Antigens, Viral (analysis), Detection, Fluorescent Antibody Technique, Human, Humans, Immunofluorescence, Microbiology, Nucleocapsid, Nucleocapsid (analysis), Pharynx (pathology), Pharynx (virology), SARS Virus (immunology), SARS Virus (isolation & purification), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (pathology), Severe acute respiratory syndrome, Severe acute respiratory syndrome virus.
- MESH :
- chemical , analysis : Antigens, Viral.
- analysis : Nucleocapsid.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- isolation & purification : SARS Virus.
- pathology : Pharynx, Severe Acute Respiratory Syndrome.
- virology : Pharynx.
- Fluorescent Antibody Technique, Humans.
Abstract
An antigen detection assay for severe acute respiratory syndrome (SARS) coronavirus was established in this study by an indirect immunofluorescence test, which utilized cells derived from throat wash samples of patients with SARS and a rabbit serum that recognized the nucleocapsid protein of SARS-associated coronavirus (SARS-CoV) but not that of other human coronavirus tested. It detected SARS-CoV in 11 of 17 (65%) samples from SARS patients as early as day 2 of illness but in none of the 10 samples from healthy controls. Compared with other diagnostic modalities for detecting SARS-CoV, this assay is simpler, more convenient, and economical. It could be an alternative for early and rapid diagnosis, should SARS return in the future.
Affiliations:
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sortKey="Chen, Shey Ying" sort="Chen, Shey Ying" uniqKey="Chen S" first="Shey-Ying" last="Chen">Shey-Ying Chen</name><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Emergency Medicine, National Taiwan University Hospital</s1><s2>Taipei</s2><s3>TWN</s3><sZ>7 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Pan Chyr" sort="Yang, Pan Chyr" uniqKey="Yang P" first="Pan-Chyr" last="Yang">Pan-Chyr Yang</name><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Department of Internal Medicine, National Taiwan University Hospital</s1><s2>Taipei</s2><s3>TWN</s3><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Chang, Shan Chwen" sort="Chang, Shan Chwen" uniqKey="Chang S" first="Shan-Chwen" last="Chang">Shan-Chwen Chang</name><affiliation wicri:level="1"><inist:fA14 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sort="Liu, I Jung" uniqKey="Liu I" first="I.-Jung" last="Liu">I.-Jung Liu</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Microbiology, College of Medicine, National Taiwan University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Cardinal Tien College of Nursing</s1><s2>Taipei</s2><s3>TWN</s3><sZ>1 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Cardinal Tien College of Nursing</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Pei Jer" sort="Chen, Pei Jer" uniqKey="Chen P" first="Pei-Jer" last="Chen">Pei-Jer Chen</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Clinical Medicine, College of Medicine, National Taiwan University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>2 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Yeh, Shiou Hwei" sort="Yeh, Shiou Hwei" uniqKey="Yeh S" first="Shiou-Hwei" last="Yeh">Shiou-Hwei Yeh</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>National Health Research Institute</s1><s2>Taipei</s2><s3>TWN</s3><sZ>3 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>National Health Research Institute</wicri:noRegion></affiliation></author><author><name sortKey="Chiang, Yu Ping" sort="Chiang, Yu Ping" uniqKey="Chiang Y" first="Yu-Ping" last="Chiang">Yu-Ping Chiang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Microbiology, College of Medicine, National Taiwan University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Li Min" sort="Huang, Li Min" uniqKey="Huang L" first="Li-Min" last="Huang">Li-Min Huang</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Department of Pediatrics, National Taiwan University Hospital</s1><s2>Taipei</s2><s3>TWN</s3><sZ>5 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Chang, Ming Fu" sort="Chang, Ming Fu" uniqKey="Chang M" first="Ming-Fu" last="Chang">Ming-Fu Chang</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Biochemistry and Molecular Biology, College of Medicine, National Taiwan University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>6 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Shey Ying" sort="Chen, Shey Ying" uniqKey="Chen S" first="Shey-Ying" last="Chen">Shey-Ying Chen</name><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Emergency Medicine, National Taiwan University Hospital</s1><s2>Taipei</s2><s3>TWN</s3><sZ>7 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Pan Chyr" sort="Yang, Pan Chyr" uniqKey="Yang P" first="Pan-Chyr" last="Yang">Pan-Chyr Yang</name><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Department of Internal Medicine, National Taiwan University Hospital</s1><s2>Taipei</s2><s3>TWN</s3><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Chang, Shan Chwen" sort="Chang, Shan Chwen" uniqKey="Chang S" first="Shan-Chwen" last="Chang">Shan-Chwen Chang</name><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Department of Internal Medicine, National Taiwan University Hospital</s1><s2>Taipei</s2><s3>TWN</s3><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Wei Kung" sort="Wang, Wei Kung" uniqKey="Wang W" first="Wei-Kung" last="Wang">Wei-Kung Wang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Microbiology, College of Medicine, National Taiwan University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Department of Internal Medicine, National Taiwan University Hospital</s1><s2>Taipei</s2><s3>TWN</s3><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of clinical microbiology : (Print)</title><title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title><idno type="ISSN">0095-1137</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of clinical microbiology : (Print)</title><title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title><idno type="ISSN">0095-1137</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigen</term><term>Antigens, Viral (analysis)</term><term>Detection</term><term>Fluorescent Antibody Technique</term><term>Human</term><term>Humans</term><term>Immunofluorescence</term><term>Microbiology</term><term>Nucleocapsid</term><term>Nucleocapsid (analysis)</term><term>Pharynx (pathology)</term><term>Pharynx (virology)</term><term>SARS Virus (immunology)</term><term>SARS Virus (isolation & purification)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (pathology)</term><term>Severe acute respiratory syndrome</term><term>Severe acute respiratory syndrome virus</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antigènes viraux (analyse)</term><term>Humains</term><term>Nucléocapside (analyse)</term><term>Pharynx (anatomopathologie)</term><term>Pharynx (virologie)</term><term>Syndrome respiratoire aigu sévère (anatomopathologie)</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Technique d'immunofluorescence</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes viraux</term><term>Nucléocapside</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Nucleocapsid</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Pharynx</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Pharynx</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Pharynx</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Pharynx</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Fluorescent Antibody Technique</term><term>Humans</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Humains</term><term>Technique d'immunofluorescence</term><term>Virus syndrome respiratoire aigu sévère</term><term>Homme</term><term>Immunofluorescence</term><term>Détection</term><term>Nucléocapside</term><term>Antigène</term><term>Microbiologie</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An antigen detection assay for severe acute respiratory syndrome (SARS) coronavirus was established in this study by an indirect immunofluorescence test, which utilized cells derived from throat wash samples of patients with SARS and a rabbit serum that recognized the nucleocapsid protein of SARS-associated coronavirus (SARS-CoV) but not that of other human coronavirus tested. It detected SARS-CoV in 11 of 17 (65%) samples from SARS patients as early as day 2 of illness but in none of the 10 samples from healthy controls. Compared with other diagnostic modalities for detecting SARS-CoV, this assay is simpler, more convenient, and economical. It could be an alternative for early and rapid diagnosis, should SARS return in the future.</div></front></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Liu, I Jung" sort="Liu, I Jung" uniqKey="Liu I" first="I.-Jung" last="Liu">I.-Jung Liu</name></noRegion><name sortKey="Chang, Ming Fu" sort="Chang, Ming Fu" uniqKey="Chang M" first="Ming-Fu" last="Chang">Ming-Fu Chang</name><name sortKey="Chang, Shan Chwen" sort="Chang, Shan Chwen" uniqKey="Chang S" first="Shan-Chwen" last="Chang">Shan-Chwen Chang</name><name sortKey="Chen, Pei Jer" sort="Chen, Pei Jer" uniqKey="Chen P" first="Pei-Jer" last="Chen">Pei-Jer Chen</name><name sortKey="Chen, Shey Ying" sort="Chen, Shey Ying" uniqKey="Chen S" first="Shey-Ying" last="Chen">Shey-Ying Chen</name><name sortKey="Chiang, Yu Ping" sort="Chiang, Yu Ping" uniqKey="Chiang Y" first="Yu-Ping" last="Chiang">Yu-Ping Chiang</name><name sortKey="Huang, Li Min" sort="Huang, Li Min" uniqKey="Huang L" first="Li-Min" last="Huang">Li-Min Huang</name><name sortKey="Liu, I Jung" sort="Liu, I Jung" uniqKey="Liu I" first="I.-Jung" last="Liu">I.-Jung Liu</name><name sortKey="Wang, Wei Kung" sort="Wang, Wei Kung" uniqKey="Wang W" first="Wei-Kung" last="Wang">Wei-Kung Wang</name><name sortKey="Wang, Wei Kung" sort="Wang, Wei Kung" uniqKey="Wang W" first="Wei-Kung" last="Wang">Wei-Kung Wang</name><name sortKey="Yang, Pan Chyr" sort="Yang, Pan Chyr" uniqKey="Yang P" first="Pan-Chyr" last="Yang">Pan-Chyr Yang</name><name sortKey="Yeh, Shiou Hwei" sort="Yeh, Shiou Hwei" uniqKey="Yeh S" first="Shiou-Hwei" last="Yeh">Shiou-Hwei Yeh</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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